mouse il 23 quantikine elisa kits  (R&D Systems)

Journal: Journal of Nanobiotechnology

Article Title: Mitochondria-targeted nanozyme system for psoriasis treatment

doi: 10.1186/s12951-026-04068-z

Figure Lengend Snippet: Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Cytokine levels in cell culture supernatants and tissue homogenates were measured using ELISA kits for IL-1β (DY401), IL-6 (M6000B), TNF-α (MTA00B), IL-17 A (DY5390), and IL-23 (M2300) (R&D Systems, USA).

Techniques: Staining, Colorimetric Assay, Activity Assay, Expressing, Marker, Enzyme-linked Immunosorbent Assay

Journal: Psoriasis: Targets and Therapy

Article Title: PEGylated Peptide for Targeted Inhibition of Human Antigen R as a Novel Therapeutic Strategy for Psoriasis: A Proof-of-Concept Study

doi: 10.2147/PTT.S563589

Figure Lengend Snippet: HIP inhibits inflammatory cytokine production and induces apoptosis in IMQ-activated Raw264.7 cells. ( a and b ) ELISA quantification of pro-inflammatory cytokines TNF-α (a) and IL-23 ( b ) in the supernatant of Raw264.7 cells. Cells were pretreated with HIP for 1 hr and subsequently stimulated with IMQ (10 μg mL −1 ) for 18 hrs. Data are presented as mean ± standard deviation (n = 4). Statistical significance was determined by Student’s t -test: *p < 0.05, **p < 0.01, ***p < 0.001 compared to the indicated control group. ( c ) Representative fluorescence microscopy images (magnification × 200) of DAPI-stained Raw264.7 cells. Images illustrate nuclear condensation, a morphological hallmark of apoptosis, following 1-hr pretreatment with HIP and 18-hr stimulation with IMQ. Scale bar = 50 μm. ( d ) Immunoblot analysis showing the cleavage of caspase-3 in total cell lysates after the indicated treatments. The histograms above the respective bands represent the relative expression levels of cleaved caspase-3 normalized to GAPDH, as quantified by densitometry using ImageJ software. Data are presented as mean ± standard deviation (n = 4). Statistical significance was determined by Student’s t -test: *p < 0.05, **p < 0.01, ***p < 0.001 compared to the indicated control group.

Article Snippet: Mouse IL-23, VEGF, and TNF-α levels were quantified using ELISA kits from R&D Systems.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation, Control, Fluorescence, Microscopy, Staining, Western Blot, Expressing, Software

Journal: Psoriasis: Targets and Therapy

Article Title: PEGylated Peptide for Targeted Inhibition of Human Antigen R as a Novel Therapeutic Strategy for Psoriasis: A Proof-of-Concept Study

doi: 10.2147/PTT.S563589

Figure Lengend Snippet: HIP alleviates IMQ-induced psoriasis-like inflammation in BALB/c mice. ( a ) Illustration of the 10-day in vivo experimental protocol involving topical application of IMQ and treatment with HIP or quercetin. ( b ) Representative macroscopic images of mouse ear skin following 10 days of IMQ application and indicated treatments. ( c – e ) Daily assessment of psoriasis-like symptoms, including erythema, scaling, and thickness, scored on a 0–4 scale. ( f ) Quantification of ear thickness using a vernier caliper. ( g ) Cumulative psoriasis severity score calculated as the sum of erythema, scaling, and thickness scores on day 10. ( h and i ) Serum levels of VEGF and IL-23 measured by ELISA on day 10. Data are presented as mean ± SEM (n = 10 per group). Statistical significance was determined by Student’s t -test (*p < 0.05, **p < 0.01, ***p < 0.001 versus the indicated control group).

Article Snippet: Mouse IL-23, VEGF, and TNF-α levels were quantified using ELISA kits from R&D Systems.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Control